Daniel:Notebook/HiResChrPaint/2013-10-23
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BSPP Control (Started 10/22/2013)
DpnII Digestion (Started 10/22/2013
3. Column Purification, elute with 30 uL EB 4. Measure concentrations in nanodrop
Nanodrop Results
TBE Gel
1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7.2 uL for all)
Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Sample | 10bp ladder | BSPP 1AB | BSPP 1CD | BSPP 2AB | BSPP 2CD |
2. Run for 23 minutes at 220V 3. Stain with 3 uL SYBR gold for 2 minutes
Gel Image