Daniel:Notebook/HiResChrPaint/2013-10-23

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Revision as of 18:38, 23 October 2013 by >Djacobse (Created page with "=BSPP Control (Started 10/22/2013)= Back to Calendar ==DpnII Digestion ([[Daniel:Notebook/HiRe...")
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BSPP Control (Started 10/22/2013)

Back to Calendar

DpnII Digestion (Started 10/22/2013

3. Column Purification, elute with 30 uL EB
4. Measure concentrations in nanodrop

Nanodrop Results

TBE Gel

1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7.2 uL for all)


Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample 10bp ladder BSPP 1AB BSPP 1CD BSPP 2AB BSPP 2CD
2. Run for 23 minutes at 220V
3. Stain with 3 uL SYBR gold for 2 minutes

Gel Image