Hosuk:LabNotes/2014-1-27
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NCIH1975 : Mutation Detection
- Continued from 1/19
2nd Rolony
- Annealing Padlock probes, Ampligase, and 2nd RCA fab. --> done at 01/19~01/21
Detection of Mutation
- Procedure
- Add Cy5-Adapt to detect 1st Rolonies, 3 positions
- Strip (80% Formamide for 15min), Add dcProbe1 (incubate 15min), image
- Strip (80% Formamide for 15min), Add dcProbe2 (incubate 15min), image
- Strip (80% Formamide for 15min), Add dcProbe3 (incubate 15min), image
- Strip (80% Formamide for 15min), Add dcProbe4 (incubate 15min), image
Result
- Image at 3 different positions
1st Rolony
- Pos1 : 5151
- Pos2 : 5964
- Pos3 : 6485