Hosuk:LabNotes/2014-1-27

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Revision as of 01:56, 28 January 2014 by >Hosuki78 (Created page with "*LabNote ===NCIH1975 : Mutation Detection=== *Continued from 1/19 ====2nd Rolony==== *Annealing Padlock probes, Ampligase, ...")
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NCIH1975 : Mutation Detection


2nd Rolony

  • Annealing Padlock probes, Ampligase, and 2nd RCA fab. --> done at 01/19~01/21


Detection of Mutation

  • Procedure
    1. Add Cy5-Adapt to detect 1st Rolonies, 3 positions
    2. Strip (80% Formamide for 15min), Add dcProbe1 (incubate 15min), image
    3. Strip (80% Formamide for 15min), Add dcProbe2 (incubate 15min), image
    4. Strip (80% Formamide for 15min), Add dcProbe3 (incubate 15min), image
    5. Strip (80% Formamide for 15min), Add dcProbe4 (incubate 15min), image


Result

  • Image at 3 different positions


1st Rolony
  • Pos1 : 5151
  • Pos2 : 5964
  • Pos3 : 6485

File:NCIH1975 Mutant Pos1-2-3 1stRolony.png