Matt:LabNotes/2014-1-24

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Revision as of 23:59, 28 January 2014 by >Mzcai (Created page with "===Generating Primary Rolonies in 32 Wells=== *Hosuk fixed PGP1F cells in 32 wells (rows C-F, columns 3-10) of 96 well plate *I followed standard procedures to generate primar...")
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Generating Primary Rolonies in 32 Wells

  • Hosuk fixed PGP1F cells in 32 wells (rows C-F, columns 3-10) of 96 well plate
  • I followed standard procedures to generate primary rolonies
    • Used half the concentration of Riboshredder because there wasn't enough left
  • Columns 3-8 used random hexamer for RT
  • Columns 9-10 used primers that are complementary to RAB7A mRNA a known distance from TSS
    • Row C: 220bp from TSS
    • Row D: 300bp from TSS
    • Row E: 510bp from TSS
    • Row F: 1000bp from TSS