Matt:LabNotes/2014-1-31

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Shifted FISSEQ vs FISSEQ_Adpt Signal Count in Same Wells

  • Previously compared the two dyes in adjacent wells Matt:LabNotes/2014-1-28
    • Here I strip FISSEQ_Adpt from before and add Shifted FISSEQ to column 9
  • Put Shifted FISSEQ_2ndRCAprimerDye in columns 4 and 9
  • Put RAB7A_1stRolonyFISH in columns 3 and 10


Well RT primer Cy3 Shifted % of Cy3
C9 220nt 711 129 0.181434599
D9 300nt 357 203 0.568627451
E9 510nt 4449 842 0.189256013
F9 1000nt 13295 14654 1.102218879
Average % of Cy3: 0.510384236

RT_RAB7A Primers and the Number of RAB7A Primary Rolonies

  • Using RAB7A_1stRolonyFISH (ATTO550)

C10_550: 24 D10_550: 24 E10_550: 13 F10_550: 35