EricChu:LabNotesMDA/2014-1-27

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Revision as of 23:08, 5 February 2014 by >Ericchu
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Redo Real Time MDA to Check Protease in MDA in PCR tubes

  • From the previous experiment, all conditions involving protease were either poorly amplified or non-amplified. Also excess (20% more) neutralization solution (HCl+TrisHCl) did not affect MDA reaction.
  • This experiment tested the adverse effect of protease or protease buffer, with heat inactivation.
  • This experiment also tested the excess of denaturation buffer in MDA in 20ul volume.
  • Since genomic DNA behaved similarly to cells, 60pg/ul human genomic DNA was used.
  • Here are the tested conditions: