EricChu:LabNotesMDA/2014-1-27
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Redo Real Time MDA to Check Protease in MDA in PCR tubes
- From the previous experiment, all conditions involving protease were either poorly amplified or non-amplified. Also excess (20% more) neutralization solution (HCl+TrisHCl) did not affect MDA reaction.
- This experiment tested the adverse effect of protease or protease buffer, with heat inactivation.
- This experiment also tested the excess of denaturation buffer in MDA in 20ul volume.
- Since genomic DNA behaved similarly to cells, 60pg/ul human genomic DNA was used.
- Here are the tested conditions: