Hosuk:LabNotes/2014-2-9

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Revision as of 06:04, 10 February 2014 by >Hosuki78 (→‎Result)
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NCIH1975 : Mutation Detection : 2nd Try, Data Analysis

  • Data from 2/3
  • Took pictures at 3 Positions on the sample in MatTek dish

Procedure

    1. Maximum projection image with all images of 8 steps
    2. Run PISA to make BW images
    3. Use the BW image as mask image file and do masking an each image (=image file .* mask image)
    4. Run PISA with masked image, and obtain a number of rolonies, centroid coordinate information of each rolony
    5. Decode the rolony to extract decoding information along steps


Major Parameters

Decode Binary table of each target
EGFR_T790M_WT EGFR_T790M_MU EGFR_L858R_WT EGFR_L858R_MU
Step1 (1FAM) 0 1 0 1
Step2 (1Cy3) 1 0 0 0
Step3 (2FAM) 0 0 1 0
Step4 (2Cy3) 0 0 0 1
Step5 (3FAM) 1 0 1 0
Step6 (3Cy3) 0 1 0 0
Step7 (4FAM) 1 0 0 1
Step8 (4Cy3) 0 1 1 0


Decode Binary String of each target
  • EGFR_T790M_WT = '01010010'
  • EGFR_T790M_MU = '10100001'
  • EGFR_L858R_WT = '10010100'
  • EGFR_L858R_MU = '01001001'


1 bit Error cases of Decode Binary String
  • EGFR_T790M_WT
    • Normal : '01010010'
    • Error 1 : '01010000'
    • Error 2 : '01000010'
    • Error 3 : '00010010'


  • EGFR_T790M_MU
    • Normal : '10100001'
    • Error 1 : '10100000'
    • Error 2 : '10000001'
    • Error 3 : '00100001'


  • EGFR_L858R_WT
    • Normal : '10010100'
    • Error 1 : '10010000'
    • Error 2 : '10000100'
    • Error 3 : '00010100'


  • EGFR_L858R_MU
    • Normal : '01001001'
    • Error 1 : '01001000'
    • Error 2 : '01000001'
    • Error 3 : '00001001'


Result

File:EGFR DecodingInfo ResultTable.png