Matt:LabNotes/2014-2-10
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Quantify ppMALAT1 in vitro Capture from 2/6/2014
Samples
- 8 PCR tubes of 35ul from 2/6/2014
- Actual volume ~30ul due to evaporation
First trial of quantifying
Procedure
- Took the first and last tube
- Brought each sample to 50ul with 20ul H2O
- Used Zymo ssDNA/RNA Clean & Concentrate Kit following standard procedure
- Eluted with 15ul H2O and quantified each with 1ul using Qubit ssDNA
Results
- Starting mass of linear ppMALAT1 in each tube: 3ul * 1434 ng/ul = 4.302 ug
- Tube1: 68.8 ng/ul
- 15ul * 68.8 ng/ul = 1032 ng = 1.032 ug
- Yield = 1.032/4.302 = 24%
- 1.032 ug * (umole/28019.4ug) = 0.0000368 umole = 36.83 pmole
- 36.83 pmole/15 ul = 2.46 pmole/ul = 2.46 uM
- Tube2: 75.3 ng/ul
- 15ul * 75.3 ng/ul = 1129.5 ng = 1.1295 ug
- Yield = 1.1295/4.302 = 26%
- same calculation as above -> 2.69 uM