EricChu:LabNotesMDA/2014-2-18

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Adding N10(unmodified) into KOH as blocking agent in v7 device

  • This experiment was done following Hosuk's Jun28 protocol (PGP1#6). The cell was pumped in ALS at 1sec interval for 10mins.
  • ALS recipe:
  1. 8ul 5M KOH
  2. 5ul 2M DTT
  3. 18.75ul 8% Tween20
  4. 10ul 100uM N10
  5. 58.25ul water
  • Final N10 concentration was 10uM.

Adding N10(unmodified) into KOH as blocking agent in Realtime MDA

  • Beside the above condition (10uM N10), 1uM and 50uM were also tested on 100pg and 10pg genomic DNA.
  • Result: N10 seemed to be inhibiting the MDA reaction. Higher concentration N10 reduced the MDA time.

File:02182014realtimeMDAN10.jpg