AlanFung:LabNotes/Rolony/2014-3-5
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Secondary Rolony Generation for Padlock Probe Concentration Test
Samples
- 96 well plate containing NCIH-1975 cells fixed by Hosuk
- Location and save location of 12 position in 96 well plate for the experiment
- High Concentration(100pM)-
Anneal Padlock Probes for 24 hours
- 1X Ampligase Buffer
- Various padlock probe concentration
- 0.5 U/ul Ampligase
- Incubate @ 45C for 24 hours
- Wrapped in parafilm and put in ziploc with wet towel
Group1 C3(1),D3(2), F3(13), C5(4)
File:Ppconcentration NCHI1975 Group1.jpg
Group2 F8(15), F5(14), F6(7), E6(8)
File:Ppconcentration NCHI1975 Group2.jpg