Kun:LabNotes/CpgSeq/2007-11-11

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Revision as of 22:27, 11 November 2007 by KunZhang (talk | contribs) (New page: ===Exp.l PCR on the captured CpG islands=== Template: two capturing reactions started at 11/09/2007. Primer: Amp_F_sol, Amp_R_sol x 1 x 2 template...)
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Exp.l PCR on the captured CpG islands

Template: two capturing reactions started at 11/09/2007. Primer: Amp_F_sol, Amp_R_sol

                          x 1         x 2
     template:            5ul         10ul
     ABI 10x buffer:    4.5ul          9ul
     25mM MgCl2:          3ul          6ul
     10mM dNTP:           1ul          2ul
     100uM AmpFSol:    0.15ul        0.3ul
     100uM AmpRSol:    0.15ul        0.3ul
     50X SYBG I:        0.4ul        0.8ul
     iTaq:                1ul          2ul
     H2O:              35.3ul       70.6ul

94C 3min -> 10 cycles of (94C 45sec -> 59C 2min -> 72C 1min) -> 12 cycles of (94C 45 sec -> 72C 1min) -> 72C 3min -> 15C hold.