Matt:LabNotes/2014-5-13

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Revision as of 23:48, 13 May 2014 by >Mzcai (Created page with "==MALAT1 Rolonies== ===Procedure=== #After fixing with 10% formalin 15min 37C & then sitting in 70% EtOH, Wash twice with 1X PBS #Add 140ul 100nM MALAT1_template to wells C2 ...")
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MALAT1 Rolonies

Procedure

  1. After fixing with 10% formalin 15min 37C & then sitting in 70% EtOH, Wash twice with 1X PBS
  2. Add 140ul 100nM MALAT1_template to wells C2 & B4
    • Pre-heat to 85C
    • Incubate @RT for 15 min
  3. Aspirate & Wash once with 1X PBS
  4. Capture with 100pM ppMALAT1
    • Incubate @45C for 24 hours
Component Volume
10X Ampligase Buffer 20
1nM ppMALAT1 20
5 U/ul Ampligase 20
H2O 140
Total 200