Matt:LabNotes/2014-5-13
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MALAT1 Rolonies
Procedure
- After fixing with 10% formalin 15min 37C & then sitting in 70% EtOH, Wash twice with 1X PBS
- Add 140ul 100nM MALAT1_template to wells C2 & B4
- Pre-heat to 85C
- Incubate @RT for 15 min
- Aspirate & Wash once with 1X PBS
- Capture with 100pM ppMALAT1
- Incubate @45C for 24 hours
Component | Volume |
10X Ampligase Buffer | 20 |
1nM ppMALAT1 | 20 |
5 U/ul Ampligase | 20 |
H2O | 140 |
Total | 200 |