Daniel:Notebook/HiResChrPaint/2014-8-12

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Revision as of 18:48, 12 August 2014 by >Djacobse (Created page with "=FISH with DAPI (Started 08/10/2014= Back to Calendar ==Hybridization== #Remove coverslip car...")
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FISH with DAPI (Started 08/10/2014

Back to Calendar

Hybridization

  1. Remove coverslip carefully and wash in pre-warmed 2X SSCT at 60C for 15 minutes
  2. Incubate in 2X SSCT at room temperature for 10 minutes
  3. Incubate in 0.2X SSC at room temperature for 10 minutes
  4. Dry with kimwipes; do not let the surface containing the cells touch the kimwipes
  5. Add 15 uL DRAQ5 (diluted 1:1000) to center of dish
  6. Cover with 22x22 #1.5 coverslip; seal with nail polish
    1. Allow at least 30 minutes for nail polish to dry
    2. Slides are quite stable for several months at 4C