Sam:LabNotes/Microbione/2009-2-5

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Revision as of 19:14, 5 February 2009 by >Sam Chiang (New page: =Follow up: Testing human 18S and Bac (E. coli) 16S primers using realtime PCR= ==Exp. Design== Template gDNA H2O -------- --- Primer: 18S-211 #1 #2 ...)
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Follow up: Testing human 18S and Bac (E. coli) 16S primers using realtime PCR

Exp. Design

Template         gDNA      H2O
                --------  ---
Primer: 18S-211  #1   #2   #3    (#1, #2 - replicates)
        18S-306  #4   #5   #6    (#4, #5 - replicates)


Results

Primer diluaiton:

Oligo tube -> add H2O (10 times nmole) uL -> 100 uM
10 uL of 100 uM (forward) + 10 uL of 100 uM (reverse) + 80 uL H2O => Primer mix 

gDNA Template dilution:

Stock solution 100 ug/mL = 100 ng/uL Oligo tube 
5 uL of 100 ng/uL + 45 uL H2O -> 50 uL of 10 ng/uL 


Discussion

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