Sam:LabNotes/Microbione/2009-2-5
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Follow up: Testing human 18S and Bac (E. coli) 16S primers using realtime PCR
Exp. Design
Template gDNA H2O -------- --- Primer: 18S-211 #1 #2 #3 (#1, #2 - replicates) 18S-306 #4 #5 #6 (#4, #5 - replicates)
Results
Primer diluaiton:
Oligo tube -> add H2O (10 times nmole) uL -> 100 uM 10 uL of 100 uM (forward) + 10 uL of 100 uM (reverse) + 80 uL H2O => Primer mix
gDNA Template dilution:
Stock solution 100 ug/mL = 100 ng/uL Oligo tube 5 uL of 100 ng/uL + 45 uL H2O -> 50 uL of 10 ng/uL