Daniel:Notebook/HiResChrPaint/2014-8-18

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Revision as of 15:04, 18 August 2014 by >Djacobse (Created page with "=Padlock Probes (Started 8/17/2014)= Back to Calendar ==USER/DpnII Digestion== '''Reaction Ta...")
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Padlock Probes (Started 8/17/2014)

Back to Calendar

USER/DpnII Digestion

Reaction Table

Reagent uL
amplicon 35
USER 5
nf H2O 40
Total 80
  1. Set up reaction according to table in a 0.2 mL PCR tube
  2. Incubate for 2 hours at 37C
  3. Add 15 uL according to DpnII Primer table (below)

DpnII Primer Table

Reagent uL
10X DpnII Buffer 10
100 uM RE-DpnII-V4 guide oligo 5
Total 15
  1. Incubate at 94C for 2 minutes
  2. Cool to 37C and incubate for 3 minutes
  3. Add 5 uL DpnII and incubate at 37C for 2 hours
  4. Heat inactivate DpnII at 65C for 20 minutes