Matt:LabNotes/2014-9-8

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Revision as of 23:25, 8 September 2014 by >Mzcai (Created page with "==in situ Padlock Probe Capture Temp: 45C vs 60C== ===Procedure=== *PGP1F Samples with 1st Rolonies prepared by Hosuk **Fixed 9/6/14 and 1st Rolonies on 9/8/14 *Add 100ul Pa...")
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in situ Padlock Probe Capture Temp: 45C vs 60C

Procedure

  • PGP1F Samples with 1st Rolonies prepared by Hosuk
    • Fixed 9/6/14 and 1st Rolonies on 9/8/14
  • Add 100ul Padlock Probe Mix
Components Volume
5 U/ul Ampligase 10
10X Ampligase Buffer 10
100uM ppCOL1A1 2
H2O 78
Total 100
  • Incubate at 37C for 30min
  • Incubate at 45C or 60C for 24hrs