Matt:LabNotes/2014-9-8
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in situ Padlock Probe Capture Temp: 45C vs 60C
Procedure
- PGP1F Samples with 1st Rolonies prepared by Hosuk
- Fixed 9/6/14 and 1st Rolonies on 9/8/14
- Add 100ul Padlock Probe Mix
Components | Volume |
5 U/ul Ampligase | 10 |
10X Ampligase Buffer | 10 |
100uM ppCOL1A1 | 2 |
H2O | 78 |
Total | 100 |
- Incubate at 37C for 30min
- Incubate at 45C or 60C for 24hrs