Matt:LabNotes/2014-9-19
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Suppressing ppCOL1A1 and other COL1A1 probes in Agi26k0gap
- Use suppressor oligos to reduce "high efficiency" padlock probes
- Reference: Deng, J. "Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming"
- Since these are 0gap probes can't use the "bridge to nowhere" strategy
- Oligos are reverse complimentary to extension arm of padlock probe and will out-compete
- Make Tm 5 degrees higher than reaction temp