Matt:LabNotes/2014-9-19

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Revision as of 01:48, 20 September 2014 by >Mzcai (Created page with "==Suppressing ppCOL1A1 and other COL1A1 probes in Agi26k0gap== *Use suppressor oligos to reduce "high efficiency" padlock probes **Reference: Deng, J. "Targeted bisulfite seq...")
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Suppressing ppCOL1A1 and other COL1A1 probes in Agi26k0gap

  • Use suppressor oligos to reduce "high efficiency" padlock probes
    • Reference: Deng, J. "Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming"
    • Since these are 0gap probes can't use the "bridge to nowhere" strategy
  • Oligos are reverse complimentary to extension arm of padlock probe and will out-compete
    • Make Tm 5 degrees higher than reaction temp