Hosuk:LabNotes/2014-10-1

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Revision as of 17:24, 2 October 2014 by >Hosuki78 (Created page with "*LabNote *Continued from 2014-09-30 *Wash with 1X PBS twice *Add 200ul 0.5uM FISSEQ_ppRCA in 2X SSC and incubate at 60C fo...")
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  • Wash with 1X PBS twice
  • Add 200ul 0.5uM FISSEQ_ppRCA in 2X SSC and incubate at 60C for 30min
  • Wash with 2X SSC twice
  • Add 200ul RCA Mix and incubate at 30C for 16hrs
    • H2O : 172uL
    • 10x Buffer : 20uL
    • dNTP : 2uL
    • a-dUTP : 4uL
    • Phi29 (1000 U/µl) : 0.5uL
  • Incubate at 30C for 20hr.