Daniel:Notebook/HiResChrPaint/2014-10-2

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Revision as of 18:13, 2 October 2014 by >Djacobse
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Hybridization Buffer Test

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I'm going to run a FISH test on several different hybridization buffers, from several protocols.

  • Beliveau Buffer (Standard)
    • 2X SSCT (0.1% Tween 20)
    • 10% dextran sulfate
    • 10 ug RNAse A
    • 50% Formamide
  • Beliveau Buffer (No Tween)
    • 2X SSC
    • 10% dextran sulfate
    • 10 ug RNAse A
    • 50% Formamide
  • Bienko Buffer
    • 5X Denhardt's solution
    • 1.7X SSC
    • 50% Formamide
    • 10% dextran sulfate
    • 50 mM Na2HPO4/NaH2PO4 buffer
  • Raj Buffer
    • 0.02% BSA
    • 2X SSC
    • 10% Formamide

Hybridization