Daniel:Notebook/HiResChrPaint/2014-10-2
Jump to navigation
Jump to search
Hybridization Buffer Test
I'm going to run a FISH test on several different hybridization buffers, from several protocols.
- Beliveau Buffer (Standard)
- 2X SSCT (0.1% Tween 20)
- 10% dextran sulfate
- 10 ug RNAse A
- 50% Formamide
- Beliveau Buffer (No Tween)
- 2X SSC
- 10% dextran sulfate
- 10 ug RNAse A
- 50% Formamide
- Bienko Buffer
- 5X Denhardt's solution
- 1.7X SSC
- 50% Formamide
- 10% dextran sulfate
- 50 mM Na2HPO4/NaH2PO4 buffer
- Raj Buffer
- 0.02% BSA
- 2X SSC
- 10% Formamide