Matt:LabNotes/2014-10-6

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Revision as of 21:37, 6 October 2014 by >Mzcai (Created page with "==Testing Agi26k0gap + suppressor Oligos & Agi26k20gap== ===Sample=== *Agi26k0gap Control: PGP1F Fix 5.19.14 S2 *Agi26k0gap + suppressors: PGP1F Fix 5.19.14 S3 *Agi26k20gap: ...")
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Testing Agi26k0gap + suppressor Oligos & Agi26k20gap

Sample

  • Agi26k0gap Control: PGP1F Fix 5.19.14 S2
  • Agi26k0gap + suppressors: PGP1F Fix 5.19.14 S3
  • Agi26k20gap: Fix 10.1.14

Procedure

  • Agi26k0gap Control
Components Volume
5 U/ul Ampligase 10
10X Ampligase Buffer 10
Agi26k0gap 35
H2O 45
Total 100
  • Agi26k0gap + suppressors
Components Volume
5 U/ul Ampligase 10
10X Ampligase Buffer 10
Agi26k0gap 35
600 nM (100nM each) supp_Mix 5
H2O 40
Total 100
  • Agi26k20gap
Components Volume
5 U/ul Ampligase 10
10X Ampligase Buffer 10
Agi26k20gap 77
25mM dNTP 1
10 U/ul KlenTaq 2
H2O 0
Total 100
  • Incubate at 37C for 30 min
  • Incubate at 60C for 24 hrs