Blue:RNA-Seq Experiments:10062014

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Revision as of 22:39, 6 October 2014 by >B1lake (Created page with "== Size Analysis of Mouse DRG Nuclei == *Recieved DRG nuclei (p2) from Lulu (Guoping Fan Lab, UCLA) on 10-03-2014 * Diluted nuclei to 600/ul, ran on biorad - one tight peak ...")
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Size Analysis of Mouse DRG Nuclei

  • Recieved DRG nuclei (p2) from Lulu (Guoping Fan Lab, UCLA) on 10-03-2014
  • Diluted nuclei to 600/ul, ran on biorad - one tight peak at 5um
  • Checked under microscope - extensive clusters
  • Filtered with 20um filter to clean up - removed many clusters - dropped [] to 250/ul
  • Measured nuclei width:
Nuclei Width (um)
6.45
9.03
6.45
5.8
9.03
5.8
4.52
7.21
5.95
5.32
7.1
7.1
6.45
5.8
7.1
7.74
5.8
5.8
5.8
5.8
7.1
5.16
5.8
8.48
10.32
7.1
5.95
5.32
5.8
7.1
9.68
6.45
Mean = 6.6971875