Blue:RNA-Seq Experiments:03062015

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Revision as of 01:10, 7 March 2015 by >B1lake
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C1 Tube Controls

  • Testing the quality of RNA for the following C1 runs:
  1. 20150205A: Naac bulk sorted nuclei (MSSM)
  2. 20130223A: GTEX Patient 10A Nuclei combined aliquots
  3. 20130223B: GTEX Patient 10B Nuclei combined aliquots
  • RNA extracted using Zymo micro RNA kit (eluted in 6ul)


RNA Concentrations:

  1. Naac Bulk sort - 18.2ng/ul
  2. GTEX 10A Bulk Sort (combined 5 aliquots) - 5.17ng/ul
  3. GTEX 10B Bulk Sort (combined 5 aliquots) - 5.10ng/ul

Ran Bioanalyzer using Pico chip and comparing to BA8 sort pool RNA ([[1]]:

media:RNA_Pico_2015-03-06_14-36-22.pdf