Daniel:Notebook/RNAFACS/2015-10-23

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Revision as of 23:32, 26 October 2015 by >Djacobse
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RNA Scope Trial Run 5: Pretreat II (Started 10/22/2015)

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Microscopy Results

Here are the microscopy results from the trial today:

For future use: using line average (when signal is strong) can reduce the amount of background noise seen. The field 1 image has the most background and the highest threshold (~50 for the Green/Yellow), whereas field 2 and field 3 only had around 20 as the intensity threshold (same gain, 750, for all fields/channels).

This is a good result. The field 1 and field 2 images show some nuclei with both FEZF2 and NeuN signal, whereas field 3 only has NeuN. We expect to see a large number of nuclei with signal in NeuN and fewer with FEZF2.