Daniel:Notebook/ComboLock/Polyacrylamide
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Polyacrylamide Gel Recipes for Combo Lock
PA gel creation recipes.
APS and TEMED
Based on the [protocol from George Church's lab for PA Gel creation on glass slides.
This recipe is done on the Drop Seq device and involves using APS and TEMED, which is a fast reaction done at room temperature.
Reagent | Stock Conc | Final Conc | Volume (uL) |
Acrylamide | 40% | 18% (9%) | 450 |
Acrylamide/Bis | 40% (19:1) | 1.9%:0.1% (0.95%:0.05%) | 50 |
dH20 | 500 | ||
Total | 1000 |