Daniel:Notebook/ComboLock/Polyacrylamide

From ZhangLabWiki
Revision as of 00:02, 12 March 2016 by >Djacobse (Created page with "=Polyacrylamide Gel Recipes for Combo Lock= Back to Notebook PA gel creation recipes. ==APS and TEMED== Based on the [[http://arep.med.harvar...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Polyacrylamide Gel Recipes for Combo Lock

Back to Notebook

PA gel creation recipes.

APS and TEMED

Based on the [protocol from George Church's lab for PA Gel creation on glass slides.

This recipe is done on the Drop Seq device and involves using APS and TEMED, which is a fast reaction done at room temperature.

Reagent Stock Conc Final Conc Volume (uL)
Acrylamide 40% 18% (9%) 450
Acrylamide/Bis 40% (19:1) 1.9%:0.1% (0.95%:0.05%) 50
dH20     500
Total     1000

VA-044