Daniel:Notebook/ComboLock/2016-3-15
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10% Acrylamide VA-044
From yesterday's results we decided to increase the acrylamide concentration in the gel solution in order to make up for a lack of formaldehyde/tissue.
VA-044/10% Acrylamide test
For test 2 we used the same setup as test 1, but with the following recipe for the VA-044 gel mix:
Reagent | Stock Concentration | Final Concentration | Amount added |
Acrylamide | 40% | 9% | 6.75 mL |
Acrylamide:Bis (19:1) | 40% (38:2) | 0.95:0.05 (%) | 0.75 mL |
VA-044 | ----- | 0.25% (m/v) | 0.075 g |
PBS | 10X | 1X | 3 mL |
ddH2O | ----- | ----- | 9.5 mL |
Total | 30 mL |
Run Parameters
- Line 1: Oil (on ice); X psi; ~2 mL
- Line 2: Aqueous (VA-044P; on ice); X psi; ~1 mL