Daniel:Protocols/CProbeLigate

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Revision as of 23:20, 5 May 2016 by >Djacobse (Created page with "=C Probe Hybridization= Back to Notebook This page describes the protocol for hybridizing C probes as used in PLAYR Design (as from [http://www.ncbi.nlm....")
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C Probe Hybridization

Back to Notebook

This page describes the protocol for hybridizing C probes as used in PLAYR Design (as from Nolan lab).

Protocol

Resuspend oligos in nuclease free water to concentration of 100 uM
    1. Fix cells
    2. Pellet cells and resuspend at concentration of ~1,000,000/mL
    3. Fix cells in medium with 1.6% paraformaldehyde for 10 min at RT
    4. Wash cells with 2X SSC buffer
    5. Pellet cells and permeabilize with ice-cold methanol for 10 min on ice