CRISPR Library Design Pipeline

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Revision as of 06:25, 21 May 2016 by >YanWu
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The process for designing the 05/05/2015 Library Order and 02/17/2016 Library Order included:

  1. Create a manually curated gene list for each library.
  2. Pick 3-4 gRNAs per gene with the highest score from this File:GRNA spacer file.txt
  3. Add scaffold sequences and desired barcodes. The barcode list can be found in grna_bc25mers.txt. The scaffold sequences can be found on this page: gRNA Scaffold Sequences.