Daniel:Notebook/ComboLock/2016-6-25

From ZhangLabWiki
Revision as of 19:28, 25 June 2016 by >Djacobse (Created page with "=Stage 2 Test (Started yesterday)= Back to Calendar ==Protocol== <ol start="7"> <li>Ethanol Precipitat...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Stage 2 Test (Started yesterday)

Back to Calendar

Protocol

  1. Ethanol Precipitation
    1. Centrifuge at 4 C for 25 minutes at 14000 rpm
    2. Remove supernatant and add 750 uL chilled 75% EtOH
    3. Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
    4. Remove supernatant and dry the pellet in the hood
    5. Resuspend pellet in 20 uL volume nfH20
    6. Measure in Nanodrop
    7. Store DNA at 4C

Nanodrop Results

Batch Source ng/uL
Batch2  
Batch3  
Batch5  
Batch6