Daniel:Notebook/ComboLock/2016-9-3

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Revision as of 21:57, 5 September 2016 by >Djacobse (Created page with "=X3 Dilution Test (Started Monday)= Back to Calendar ==Protocol (from [[Daniel:Notebook/ComboLock/2016-...")
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X3 Dilution Test (Started Monday)

Back to Calendar

Protocol (from yesterday)

  1. Ethanol Precipitation
    1. Pellet by centrifuging for 25 minutes at 12000rpm at 4C
    2. Remove supernatant and add 750 uL chilled 70% EtOH
    3. Centrifuge for 12 minutes at 12000rpm at 4C
    4. Remove supernatant and spin in vacuum centrifuge for 10 minutes
    5. Add 20 uL nfH2O and measure in nanodrop
    6. Nanodrop Results

      Since these results aren't great, I'm going to try again with size select on Monday.

      Sample ng/uL
      Sample A 2.5
      Sample B 5.9