Matt:LabNotes/2016-11-15

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In vitro capture with CA12kOct2016 V4 SplintR Ligase Test 2

  • gDNA and UHRR
  • SplintR and T4 DNA Ligase for HBRR

Reference

Experimental Outline

  1. CA12kOct2016 V4 Padlock Probe capture of gDNA and RNA
  2. Sequence to quantify probe efficiencies

Sample Groups

  • RNA template = (30ng or 150ng) Universal Human Reference RNA (UHRR 740000-41)
  • DNA template = 300ng gDNA 12878
  1. NTC - 8.19ng V4 - SplintR
  2. 30ng RNA - 8.19ng V4 - SplintR
  3. 150ng RNA - 40.9ng V4 - T4
  4. 300ng gDNA - 16.38ng V4 - Ampligase

Experiment