Matt:LabNotes/2016-11-15
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In vitro capture with CA12kOct2016 V4 SplintR Ligase Test 2
- gDNA and UHRR
- SplintR and T4 DNA Ligase for HBRR
Reference
Experimental Outline
- CA12kOct2016 V4 Padlock Probe capture of gDNA and RNA
- Sequence to quantify probe efficiencies
Sample Groups
- RNA template = (30ng or 150ng) Universal Human Reference RNA (UHRR 740000-41)
- DNA template = 300ng gDNA 12878
- NTC - 8.19ng V4 - SplintR
- 30ng RNA - 8.19ng V4 - SplintR
- 150ng RNA - 40.9ng V4 - T4
- 300ng gDNA - 16.38ng V4 - Ampligase