Daniel:Notebook/ComboLock/2016-11-21

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Revision as of 20:51, 21 November 2016 by >Djacobse (Created page with "=Sequencing Results-Miseq 2016-11-17= Back to Calendar ==Circularization Optimization Testing (Started [[Daniel:Notebook/ComboLock/2016-10-31|O...")
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Sequencing Results-Miseq 2016-11-17

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Circularization Optimization Testing (Started Oct 31)

This was a repeat of the sequencing from 11-10, for which the results are analyzed on Daniel:Notebook/ComboLock/2016-11-18) The base qualities and results were not great, so I tried to repeat it. However, we can see:

that the base qualities are all poor. Further, of the few sequences that actually aligned to the template, none of them are sufficiently out of the AmpF region (approximately bp 56). To make this calculation you can take the alignment position in column 4 and compare it against the number of matches in the CIGAR string (column 6) and see that 59 is about as high as it gets, which isn't necessarily far enough into the next region to determine its identity.

Cell Test with RCA (Started Nov 7)