Daniel:Notebook/ComboLock/2017-1-30
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RCA Test (Started Monday)
Library Prep (Started Jan 26)
- Ethanol Precipitation
- Centrifuge for 30 minutes at 12500 rpm at 4C
- Remove supernatant and add 750 uL chilled 70% EtOH
- Centrifuge for 15 minutes at 12500 rpm at 4C
- Remove supernatant; dry in vacuum centrifuge for 15 minutes
- Resuspend in 20 uL H2O and consolidate samples
- Measure with Qbit
- Consolidate with samples from Thursday 1-26
DEJ20160126-CLv3-RCA02
Final Sample Matrix
Sample | Sample Amt | Pre/Post RCA | AmpR Index |
Sample 0 (AB) | 1 pmol | Pre | 21 |
Sample 0X (AB) | 1 pmol | Post | 25 |
Sample 2X (AB) | 10 fmol | Post | 26 |
Sample 4X (AB) | 100 amol | Post | 27 |
Sample 6C (AB) | 1 amol | Epi | 22 |
Sample 6D (AB) | 1 amol | Epi 1:10 | 23 |