Daniel:Notebook/ComboLock/2017-1-30

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Revision as of 23:25, 30 January 2017 by >Djacobse (→‎Library Prep (Started Jan 26))
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RCA Test (Started Monday)

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Library Prep (Started Jan 26)

  1. Ethanol Precipitation
    1. Centrifuge for 30 minutes at 12500 rpm at 4C
    2. Remove supernatant and add 750 uL chilled 70% EtOH
    3. Centrifuge for 15 minutes at 12500 rpm at 4C
    4. Remove supernatant; dry in vacuum centrifuge for 15 minutes
    5. Resuspend in 20 uL H2O and consolidate samples
    6. Measure with Qbit
    7. Consolidate with samples from Thursday 1-26

DEJ20160126-CLv3-RCA02

Final Sample Matrix

Sample Sample Amt Pre/Post RCA AmpR Index
Sample 0 (AB) 1 pmol Pre 21
Sample 0X (AB) 1 pmol Post 25
Sample 2X (AB) 10 fmol Post 26
Sample 4X (AB) 100 amol Post 27
Sample 6C (AB) 1 amol Epi 22
Sample 6D (AB) 1 amol Epi 1:10 23