Daniel:Notebook/ComboLock/2017-3-27

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Revision as of 16:14, 27 March 2017 by >Djacobse (Created page with "=RCA Test (Started March 24)= Back to Calendar ==Protocol== <ol> <li>Rolling Circle Amplification</li>...")
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RCA Test (Started March 24)

Back to Calendar

Protocol

  1. Rolling Circle Amplification
    1. Add 20 uL nfH2O to every sample
  2. qPCR
    1. Make master mixes according to following table
    2. Reagent 1X Rxn MM-Amp (33.2X) MM-Prod (9.2X)
      Primer Pair NA AmpF/AmpR p4/p12RC
      Forward Primer (10 uM) 1 33.2 9.2
      Forward Primer (10 uM) 1 33.2 9.2
      Kapa SYBR Fast Master Mix (2X) 25 830 230
      nfH2O 21 697.2 193.2
      Total 48 1593.6 441.6