Daniel:Notebook/ComboLock/2017-3-27
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RCA Test (Started March 24)
Protocol
- Rolling Circle Amplification
- Add 20 uL nfH2O to every sample
- qPCR
- Make master mixes according to following table
Reagent | 1X Rxn | MM-Amp (33.2X) | MM-Prod (9.2X) |
Primer Pair | NA | AmpF/AmpR | p4/p12RC |
Forward Primer (10 uM) | 1 | 33.2 | 9.2 |
Forward Primer (10 uM) | 1 | 33.2 | 9.2 |
Kapa SYBR Fast Master Mix (2X) | 25 | 830 | 230 |
nfH2O | 21 | 697.2 | 193.2 |
Total | 48 | 1593.6 | 441.6 |