Daniel:Notebook/PosSequencing/2017-4-14
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Bead Loading Analysis (Started Tuesday)
Image Analysis (Started Wednesday)
Analysis of the images taken on Wednesday. Since there are only 6 and I'm not planning on doing this long, I did the analysis manually. Here's the pipeline, done in Powerpoint.
- Start with Dapi image and identify (by eye) the bright clusters; circled in red File:20170412-D1Emulsion-DAPI3-Analysis.png
- Use "change image function" to replace DAPI image with brightfield image. The change image function keeps the position and dimensions of the original
- Mark and count (in blue) the beads that appear in the same plane as the red circled dots but had no signal. These are confirmed no-calls. The green circled dots are "wild cards", and are possibly no-calls, although they might just be out of phase which lowers there signal File:20170412-D1Emulsion-BF3-Analysis.png