Daniel:Notebook/PosSequencing/2017-4-14

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Revision as of 21:10, 14 April 2017 by >Djacobse (Created page with "=Bead Loading Analysis (Started Tuesday)= Back to Calendar ==Image Analysis (Started [[Daniel:N...")
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Bead Loading Analysis (Started Tuesday)

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Image Analysis (Started Wednesday)

Analysis of the images taken on Wednesday. Since there are only 6 and I'm not planning on doing this long, I did the analysis manually. Here's the pipeline, done in Powerpoint.

  1. Start with Dapi image and identify (by eye) the bright clusters; circled in red
  2. File:20170412-D1Emulsion-DAPI3-Analysis.png
  3. Use "change image function" to replace DAPI image with brightfield image. The change image function keeps the position and dimensions of the original
  4. Mark and count (in blue) the beads that appear in the same plane as the red circled dots but had no signal. These are confirmed no-calls. The green circled dots are "wild cards", and are possibly no-calls, although they might just be out of phase which lowers there signal
  5. File:20170412-D1Emulsion-BF3-Analysis.png