Daniel:Notebook/ComboLock/2017-5-2
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Production Run (Started Friday 4/28)
Library Prep
- Qiaquick Purification
- Combine 20 uL each sample into a 1.5 mL tube following matrix
- Add 200 uL (5X) PB (binding buffer) to sample
- Load sample onto column and spin for 1 minute at 14000 rpm; discard flow through
- Add 750 uL PE (wash buffer) to column and spin for 1 minute at 14000 rpm; discard flow through
- Dry spin column for 1 minute at 14000 rpm; discard flow through
- Let stand with cover open in fume hood for ~5 minutes
- Transfer column to a new 1.5 mL eppendorf tube
- Add ≥30 uL EB to column
- Let stand 1 minute
- Spin for 1 minute at 14000 rpm
- Size Select TBE Gel
Tube | Sample 1 | Sample 2 | Indexes |
1X | 1AX | 1BX | 20 |
5AX | 5AX | nfH2O | 24 |
1Y | 1AY | 1BY | 25 |
5AY,4AY | 5AY | 4AY | 26,27 |