Daniel:Notebook/ComboLock/2017-5-2

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Revision as of 16:33, 2 May 2017 by >Djacobse (Created page with "=Production Run (Started Friday 4/28)= Back to Calendar ==Library Prep== <ol start="10"> <li>Qiaquick ...")
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Production Run (Started Friday 4/28)

Back to Calendar

Library Prep

  1. Qiaquick Purification
    1. Combine 20 uL each sample into a 1.5 mL tube following matrix
    2. Tube Sample 1 Sample 2 Indexes
      1X 1AX 1BX 20
      5AX 5AX nfH2O 24
      1Y 1AY 1BY 25
      5AY,4AY 5AY 4AY 26,27
    3. Add 200 uL (5X) PB (binding buffer) to sample
    4. Load sample onto column and spin for 1 minute at 14000 rpm; discard flow through
    5. Add 750 uL PE (wash buffer) to column and spin for 1 minute at 14000 rpm; discard flow through
    6. Dry spin column for 1 minute at 14000 rpm; discard flow through
    7. Let stand with cover open in fume hood for ~5 minutes
    8. Transfer column to a new 1.5 mL eppendorf tube
    9. Add ≥30 uL EB to column
    10. Let stand 1 minute
    11. Spin for 1 minute at 14000 rpm
  2. Size Select TBE Gel