Daniel:Notebook/PosSequencing/2017-5-11
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Bead Binding Test 2 (Started Yesterday)
Image Processing
- Open new image in photoshop; use resolution 1344x1024 (same as .jpg images from microscope)
- Import brightfield and DAPI images to photoshop file
- Adjust levels as deemed useful using Image->Adjustments->Levels
- Create new red solid color layer
- Click on the mask icon (image next to red) and then go to Image->Apply Image...
- Use DAPI image as "Source" and choose "Multiply" under "Blending"
- Hide the original DAPI layer and save blended image
Results
<gallery perrow=2 heights=300px widths=300px caption="DAPI overlays for Sample 1 prior to melting"> File:|Field File:Sample1-dsDNA-Overlay-F2-Blend.png|Field 2 <gallery>
<gallery perrow=3 heights=300px widths=300px caption="DAPI overlays for Sample 2 prior to melting"> File:Sample2-dsDNA-Overlay-F1-BlendAdj.png|Field 1 File:Sample2-dsDNA-Overlay-F2-BlendAdj.png|Field 2 File:|Field 3 <gallery>