Daniel:Notebook/PosSequencing/2017-7-12
Jump to navigation
Jump to search
emPCR8 (Started Thursday July 6)
Protocol (From Yesterday)
- Dye hybridization
- Mix 20 uL ssDNA (lambda digested) and 20 uL 4X SSC buffer
- Incubate in the dark at 70C for 5 minutes
- Incubate at 55C for 20 minutes
- Add 1 uL appropriate (10 uM) dye, A488-dc6 for sample 1 and Cy5-dc6 for sample 3
- Incubate in the dark (RT) for 15 minutes
- Magnet pulldown, remove supernatant and add 40 uL 2X SSC buffer
- Add 15 uL to a slide for analysis
- Seal coverslip with nail polish and wait approximately 30 minutes to dry
- 20170712-emPCR8 Barcode3-Field4-SNAP ch00.jpg
Field 4
- 20170712-emPCR8 Barcode3-Field5-SNAP ch00.jpg
Field 5
- 20170712-emPCR8 Barcode3-Field6-SNAP ch00.jpg
Field 6
- 20170712-emPCR8 Barcode-v1v3-Field1-SNAP ch00.jpg
Field 1
- 20170712-emPCR8 Barcode-v1v3-Field2-SNAP ch00.jpg
Field 2
- 20170712-emPCR8 Barcode-v1v3-Field5-SNAP ch00.jpg
Field 5
- 20170712-emPCR8 Barcode-v1v3-Field6-SNAP ch00.jpg
Field 6