Kun:LabNotes/ASE/2007-12-14
Jump to navigation
Jump to search
Exp.1 PCR on the circularized CES22k probes
one reaction 12 reactions Template: 10ul each 10x Buffer: 9ul 108ul 25mM MgCl2 5.4ul 64.8ul 25mM dNTP 1.2ul 13.2ul 100uM AmpSolV6F2 0.4ul 4.8ul 100uM AmpSolV6R2 0.4ul 4.8ul JumpStart Taq 1ul 12ul 50X SYBG I 0.8ul 9.6ul H2O 72ul 861ul
94C 3min -> 8 cycles of (94C 45sec -> 57C 1min -> 72C 1min) -> 10 cycles of (94C 45 sec -> 72 1min) -> 72C 3min -> 15C hold.
Took out 1C and 2A, add 4 more cycles on the remaining reactions.