Jie:LabNotes/CpgSeq/2009-6-22
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Methyl_PCR for ASE validation
PCR on bisuphite conversed Jurkat-DNA
2x iQ supermix 20 x 15 Primer F (50uM) 0.5 0.5 each Primer R (50uM) 0.5 0.5 each BS Jurkat gDNA (202ng/ul) 0.1 1.5 dH2O 20 300 total 40ul
The primers are plate2_A1-H1. I set the annealing temperature at 55C, 58C, 62C. For iQ supermix: 96C 3min-> 40x (95C 30sec, 62C 1min, 72C 1min)-> 72C 5min.