Jie:LabNotes/CpgSeq/2009-6-22

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Revision as of 17:02, 22 June 2009 by >Jie deng (New page: ==Methyl_PCR for ASE validation== ===PCR on bisuphite conversed Jurkat-DNA=== 2x iQ supermix 20 x 15 Primer F (50uM) 0.5 0.5 ea...)
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Methyl_PCR for ASE validation

PCR on bisuphite conversed Jurkat-DNA

2x iQ supermix                         20             x   15          
Primer F (50uM)			0.5	        0.5 each
Primer R (50uM)			0.5             0.5 each
BS Jurkat gDNA (202ng/ul)     	        0.1	  	1.5
dH2O				         20		300
total				         40ul
The primers are plate2_A1-H1. I set the annealing temperature at 55C, 58C, 62C. 
For iQ supermix: 96C 3min-> 40x (95C 30sec, 62C 1min, 72C 1min)-> 72C 5min.