Jie:LabNotes/CpgSeq/2009-6-22

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Revision as of 16:57, 23 June 2009 by >Jie deng
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Methyl_PCR for ASE validation

PCR on bisuphite conversed Jurkat-DNA

2x iQ supermix                         20             x   12          
Primer F (50uM)			0.5	        0.5 each
Primer R (50uM)			0.5             0.5 each
BS Jurkat gDNA (202ng/ul)     	        0.1	  	1.2
dH2O				         20		240
total				         40ul
The primers are 20061015_ASM_valid. I set the annealing temperature at 58C, 62C. 
96C 3min-> 40x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min.

File:20090622 ASM 58C anneal.jpg20090622_ASM_58C anneal

File:20090622 ASM 62C anneal.jpg20090622_ASM_62C anneal