Kun:LabNotes/ASE/2007-12-31

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Exp.1 Make gDNA-free cDNA from GM20431, GC1EP and Hues6

                  GM20431(06/04/07)   GC1EP(06/14/07) Hues6(05/14/07)
   total RNA       1.25ug/ul x8ul     645ng/ul x 15ul  270ng/ul x 25ul
   10x buffer         3ul                 3ul               3ul
   H2O (DEPC)        17ul                10ul               0ul
   DNase I(2u/ul)     2ul                 2ul               2ul
   RNase Inhibitor  0.5ul               0.5ul             0.5ul
   37C 30min -> 75C 10min -> Cleanup with RNeasy columns

Exp.2 Also process RNAs from Hybrid1, Hybrid2, BJ Fibroblast

                     Hybrid1            Hybrid2         BJ Fibroblast
   total RNA       1.9ug/ul x8ul     570ng/ul x 20ul   72ng/ul x 52ul
   10x buffer         3ul                 3ul               6ul
   H2O (DEPC)        17ul                 5ul               0ul
   DNase I(2u/ul)     2ul                 2ul               2ul
   RNase Inhibitor  0.5ul               0.5ul             0.5ul
   37C 30min -> 75C 10min -> Cleanup with RNeasy columns