Kun:LabNotes/ASE/2007-12-31
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Exp.1 Make gDNA-free cDNA from GM20431, GC1EP and Hues6
GM20431(06/04/07) GC1EP(06/14/07) Hues6(05/14/07) total RNA 1.25ug/ul x8ul 645ng/ul x 15ul 270ng/ul x 25ul 10x buffer 3ul 3ul 3ul H2O (DEPC) 17ul 10ul 0ul DNase I(2u/ul) 2ul 2ul 2ul RNase Inhibitor 0.5ul 0.5ul 0.5ul 37C 30min -> 75C 10min -> Cleanup with RNeasy columns
Exp.2 Also process RNAs from Hybrid1, Hybrid2, BJ Fibroblast
Hybrid1 Hybrid2 BJ Fibroblast total RNA 1.9ug/ul x8ul 570ng/ul x 20ul 72ng/ul x 52ul 10x buffer 3ul 3ul 6ul H2O (DEPC) 17ul 5ul 0ul DNase I(2u/ul) 2ul 2ul 2ul RNase Inhibitor 0.5ul 0.5ul 0.5ul 37C 30min -> 75C 10min -> Cleanup with RNeasy columns