AlanFung:Protocol/Genome Analyzer

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Revision as of 14:06, 21 July 2009 by >Alan6017518 (→‎image control)
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Genome Analyzer User Guide

SCS v2.4

  • start up scsv2.4

setting the run parameters

  • click <view>
  • clear box next to show on start up
  • from the data collection screen
  • edit|run parameters|GA Settings / RunBrowser Settings

GA Setting

  • specify the network location for run results
  • set perferences for real time analysis results
  • set settings for auto calibration
*delete images (select this option to delete images from the genome analyzer computer after analysis

Validation strings

  • edit if you are using a customized identification system

Use auto calibration

  • select this option to enable the auto calibration feature
  • refer to page 29 for a description of the auto calibration feature
  • note: if this option is cleared, focus calibration must be performed manually as described in appendix B

Run Browser Settings

  • runbrowzer reports, specify which runbrowser reports you want to show and export
if selected the first cycle is generated by the software
  • enable runbrowser report emails to sens reports to the email addresses specified
  • after you have finished selecting the settings in the run parameters window, click <save & continue>/<cancel & continue>/<reset default configs>

initialize the software

  • click the manual control/setup tab
  • perform any operation to trigger the genome analyzer initialization

run window

recipe tab

  • hover over a stop to show the parameters specific to that step

image cycletab

white tile are queued for imaging
blue tiles haven been imaged
gray tiles are not defined in the current run
right side of the window shows the photographs being taken of the current tile

temperature tab

  • for monitor various run temperatures

manual control/setup window

manual control

  • settings used for manual focus calibration

pump tab

command
to
solution
volume(ml)
aspirate rate(ml/min)
dispense rate(ml/min)

recipe viewer

  • genome analyzer recipes found can be found in
<install directory>\datacollection_v<#>\bin\recipes
  • to access the recipe view click file|open recipe from the toolbar
  • the following information is listed for each recipe
recipe
subfolder
cycle
read end
multiplexing
service
version
comment 
file status

editing cycle number

  • click edit on the recipe viewer toolbar

reagent tracking

  • provides email notifications and warnings when reagents are getting low and need replacing
  • beneficial when performing runs longer than 36 cycles
  • click reagents from the toolbar at the top of the data collection software screen

current volumes

reset volumes

reagent barcodes

  • to track reagents for any given run
barcodes are stored in the Reagentsld.xml file in the runfolder
  • if the remaining reagents are not to be used click clear

configure tracking

  • to enable or disable reagent tracking features select reagents|configure tacking
enable volume tracking
autostop when empty
display and email warnings

disk space checking

  • during the run at the end of each cycle the software checks available disk space
  • if there is insufficient disk space to accommodate one cycle the software pauses the run and a dialog box appears to tell you how much dish space remains. when sufficient disk space is available the dialog box closes and the run automatically resumes

image control

zoom in
move the zoomed image
zoom out
modify the color display
view or hide the center mark
see intensity values
see focus quality and uniformity 
show region of interest
save the image

auto calibration

Cluster Station