Kun:LabNotes/ExonomeSeq/2008-1-4
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Colony PCR
Perform PCR on 48 colonies from E55kv1(250+450) for sequencing
2X Taq master mix: 15ul x 50 750 10uM M13 primer mix: 1ul 50 H2O: 14ul 700
94C 3min -> (94C 30sec -> 54C 30sec -> 72C 45sec) x 35 -> 72C 3min -> 4C hold.