Kun:LabNotes/ExonomeSeq/2008-1-4

From ZhangLabWiki
Revision as of 23:02, 4 January 2008 by KunZhang (talk | contribs) (New page: ==Colony PCR== Perform PCR on 48 colonies from E55kv1(250+450) for sequencing 2X Taq master mix: 15ul x 50 750 10uM M13 primer mix: 1ul 50 H2O: ...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Colony PCR

Perform PCR on 48 colonies from E55kv1(250+450) for sequencing

    2X Taq master mix:    15ul  x 50  750
    10uM M13 primer mix:   1ul         50
    H2O:                  14ul        700

94C 3min -> (94C 30sec -> 54C 30sec -> 72C 45sec) x 35 -> 72C 3min -> 4C hold.