AlanFung:Protocol/Qubit
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Qubit Fluorometer Protocol
Samples & Materials
- Qubit Assay Tubes (500, Invitrogen Cat. no.Q32856) or Axygen PCR-05-C tubes (VWR, part number 10011-830)
Calibrating the Qubit Fluorometer
Running a New Calibration
- Insert standard #1
- Press <Go>
- Wait for ~5s
- Insert standard #2
- Press <Go>
Using the Last Calibration
- Highlight <Use Last Calibration>
- Press <Go>
Reading Samples
- Insert sample
- Press <Go>
- Read the result on the display
- Record the reading
- Remove the sample form the instrument
- Insert the next sample
- Press <Go>
- Repeat until all samples have been read