AlanFung:Protocil/Cluster Station
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Performing linearization, blocking , and primer hybridization
wash the cluster station
- select file|open recipe
- open the standard protocol recipe and click run
- install the washing bridge and load containers filled with water in the following positions
*single-read recipe-load position 3,4,7,12,15,17 *paired-end recipe-load position 3,4,7,12,14,17
- click ok to proceed and follow the prompts when the wash is complete
- remove water from the following positions
*single-read recipe-load position 3,4,7,12,15,17 *paired-end recipe-load position 3,4,7,12,14,17
- click ok and follow the prompts when the air gap has been primed
loading reagents
best practices always remove and replace reagents one tube at a time wear gloves at all times do not touch reagents with bare hands connect the tubes by holding the caps stationary while you twist the tubes into place to prevent crimping and twisting of the lines