HUES

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Revision as of 15:54, 24 July 2009 by >Alan6017518 (→‎Materials and Reagents)
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HUES Cell Collection

HUES homepage

  • HUES homepage[1]

Protocol

Storage

  • Location:Dr. Varghese lab LN tank Rack #2 Box "Zhang Lab"

HUES Cell Log

  • 1 x HUES-3 p22
  • 1 x HUES-8 p19
  • 1 x HUES-53 p11
  • 1 x HUES-62 p10
  • 1 x HUES-64 p12
  • 1 x HUES-66 p12
  • 2 x PMEFi

Materials and Reagents

Materials / Catalogue no. ' '
KO‐DMEM Invitrogen Gibco CAT#10829
DMEM Invitrogen Gibco CAT#11965‐092
FBS Va lley Biomedical, Inc CAT#BS‐3033
KO‐Serum Replacenment Invitrogen Gibco CAT#10828
Plasmanate Bayer CAT#NDC 0026‐0613‐20
bFGF Invitrogen Gibco CAT#13256‐029
hLIF Chemicon International CAT#LIF1010
2‐beta‐mercaptoethanol Gibco CAT#21985‐023
Non‐essential amino acids (NEAA) Invitrogen Gibco CAT#11140050
(10mM or 100x)
Pen/Strep Invitrogen Gibco CAT#15070‐063
(5000U/ml Penicillin G sodium and
5000ug/ml Streptomycin sulfate in 0.85% saline)
Glutamax‐I Invitrogen Gibco CAT#35050‐061
(200mM or 100x)
Mitomycin C Sigma CAT#M‐0503
0.05% Trypsin/EDTA Invitrogen Gibco CAT#25300‐054
Dimethyl sulphoxide (DMSO) Sigma CAT#D2650
Gelatin Sigma CAT#G1890
Tissue Culture Treated Plates (6 well) Coring CAT#3506
Hygromycin resistant, pre-treated MEFs Specialty Media CAT#PMEF‐H
Neomycin resistant, pre-treated MEFs Specialty Media CAT#PMEF‐N

Contact Information

HUES Cell Facility Contact Information

  • HUES Facility / Melton Laboratory / HHMI
  • Harvard University
  • Cambridge, MA 02138
  • Fax: 617.384.9463
  • Please send all HUES cell correspondence to hues@mcb.harvard.edu.

Harvard's Office of Technology Development Contact Information

  • Harvard's Office of Technology Development
  • 1350 Massachusetts Avenue
  • Holyoke Center 727
  • Cambridge, MA 02138
  • Fax: 617.495.9568
  • Please send all correspondence concerning the MTA to Material Transfers: materialtransfer@harvard.edu.