Jie:LabNotes/CpgSeq/2009-7-27

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Revision as of 18:35, 27 July 2009 by >Jie deng (New page: ==MMP9 ASE of PGP1F and PGP1L== ===PCR=== The primers x 6 2x iQ supermix 40 240 Primer F (100uM) ...)
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MMP9 ASE of PGP1F and PGP1L

PCR

The primers 
                                           x 6
2x iQ supermix                40           240         
Primer F (100uM)             0.4           2.4 each
Primer R (100uM)             0.4           2.4 each
cDNA samples as above:       0.1	  	
dH2O                          40           240
total                         80ul

96C 3min-> 45x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min.