Kun:LabNotes/ExonomeSeq/2008-1-16
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Exp.1 Perform another round of exon capture experiment
NA10835(347ng/ul) 3ul 3ul E55kv1 probes 180nM (01/12/08,purified) 10ul 275nM 7ul 10X Ampligase buffer 1.5ul 1ul
95C 10min -> 60C 40h -> add 2.5ul NSL mix (Stoffel 1U/ul, AmpLigase 0.5U/ul, dNTP 5uM) -> 60C 3h - -> 37C 1min -> add 1ul Exo I&&III, 1ul T7 exo, 37C 2h -> 94C 5min -> 4C hold.