Kun:LabNotes/CpgSeq/2008-1-15

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Revision as of 02:09, 18 January 2008 by KunZhang (talk | contribs) (New page: ==CloneWell purification of CpGMIP capture-PCR product== My hypothesis is that some CpG islands were captured, but didn't show up in the gel of PCR products because the self-circularized c...)
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CloneWell purification of CpGMIP capture-PCR product[edit]

My hypothesis is that some CpG islands were captured, but didn't show up in the gel of PCR products because the self-circularized circles dominated the reactions. If this is true, I can use clonewell to purify the invisible bands using the positive control band as reference, then do another round of PCR to re-amplify the captured CpG islands.