Kun:LabNotes/CpgSeq/2008-1-17
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PCR on the clonewell purified DNA[edit]
Template: ~5ul 2X iProof Master Mix: 25ul 100uM AmpSolF: 0.2ul 100uM AmpSolR: 0.2ul 50X SYBG I: 0.2ul H2O: 19.5ul 98C 30sec -> 20 cycles of (98C 10sec -> 68C 30sec) -> 68C 1min -> 15CF hold