Sam:LabNotes/Microbiome-new/2009-8-11

From ZhangLabWiki
Revision as of 00:41, 7 August 2009 by >Sam Chiang (New page: ='''E-gel size selection for mouse microbiome PCR product'''= ==Background== *Unspecific PCR amplicon leads to sequencing failure in previous Genewiz results. *Review all of previous c...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

E-gel size selection for mouse microbiome PCR product

Background

  • Unspecific PCR amplicon leads to sequencing failure in previous Genewiz results.
  • Review all of previous candidates PCR amplicons. Perform E-gel size selection for band purification.


E-gel 2% agarose gel size selection

  • Prepare the clean PCR tubes for collection
  • Set up E-gel machine. Open a new gel and remove the comb. Assemble the gel into the machine.
  • Load 20uL PCR products into each of the upper row wells. Load 6uL diluted ladder in the "M"-well. filled up the empty well with 20uL diH2O (low quality H2O is fine)
  • Fill up 20uL of nuclease-free H2O in the (lower row) collecting wells and 6~10 uL H2O for the "M" well.